Serum
Free
Media
for
Bulk
Culture
of
Hybridoma
Cells - The
media
for
culturing
a
variety
of
animal
cells
and
discussed
the
significance
of
adding
serum
to
basal
nutrient
media.
Serum
is
a
highly
complex
and
poorly
defined
mixture
of
components
like
albumin,
transferrin,
lipoproteins
and
various
hormones/growth
factors.
Nevertheless,
serum
makes
an
essential
component
of
media
for
culturing
animal
cells.
The
use
of
serum,
however,
leads
to
difficulties
in
purification
of
antibodies.
Furthers,
it
is
an
expensive
technology
for
large
scale
production
of
hybridoma
cells
for
industrial
production
of
monoclonal
antibodies.
In
view
of
these
difficulties,
serum
free
media
are
being
increasingly
used
for
culturing
hybridoma
cells.
