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Ig Gene in Germline and Differentiated Cells -
By using cDNA probes for different regions of an immunoglobulin, it was possible to demonstrate that in the antibody producing cells the DNA sequences coding for C and V regions of an Ig gene are very close to each other, though not contiguous. On the other hand, in the germline these sequences are separated from each other, sometimes by long distances.

This was expected because in earlier studies the same C region was found combined with different V regions in the same individual. Later experiments showed that C-C splicing at the DNA level is responsible for generating the coding genes for both light and heavy chains.

It was also shown that although the genes for an immunoglobulin molecule may be found on more than one chromosome, but cutting and rejoining of DNA sequences takes place only within a chromosome and not across chromosomes.

In addition to C and V sequences, both light and heavy chain coding sequences have another sequence called J (junction or joining sequences). In heavy chain sequence, in addition to C, J and V sequences, another short sequence D ( diversity) is also found.

There is also a debate on the safety of the use of the products of biotechnology, an area described as biosafety. Among application, on the other hand, efforts are also being made to use biotechnology to protect the environment from pollution and to conserve natural resources. At a time, when the gap between those who have plenty and those who do not have even the minimum is widening, both ends of this spectrum, i.e. plenty and poverty, are contributing to environmental degradation. It is, therefore, necessary that the developing and developed countries jointly find a path of development which meets the needs of the present without compromising the ability, of future generations to meet their needs (World Commission on Environment and Development). Efforts are being made to achieve this objective through a variety of approaches, and biotechnology is certainly one of them. In this chapter and the next four chapters, environmental; implications and applications of biotechnology for environment will be discussed.

In recent years, we have witnessed a debate on the environmental implications of biotechnology. In this debate, risks involved in the use of biotechnological approaches have often been emphasized (or even overemphasized) and the adequate guidelines for safety have been suggested ad enforced by law. However, there have also been rapid developments in the applications of biotechnology, which may help in controlling environment pollution, thus giving a cleaner and sustainable environment in future. According to one estimate in USA, the US market for environmental clean-up applications was expected to grow at an average rate of 17%, while that for microbes and enzymes was expected to grow by only 7% every year.

Besides others, these applications for environment clean-up include biotreatment methods for effluents and toxic wastes (this subject is described as bioremediation and is discussed in the next). However, these bioremediation treatments, it is feared, could be problematic, where they involve deliberate or accidental release of genetically modified microbes to the environment. These applications of biotechnology in environment management.