Disease/pathogen |
Method of attenuation |
| 1. Rabies |
Cultivation in chick or duck embryo |
| 2. Yellow fever |
Cultivation in chick embryo |
| 3. Smallpox |
Animal passage (calf); genetic engineering |
| 4. Polio |
Mutant strain (sabin oral polio vaccine) |
| 5. RCG. (Bacillus of Chalmette Gucrien) |
Cultivation of organism on media containing bile |
| 6. Bacillus anthracis |
Cultivation at 40-50°C |
