Affinity Chromatography - Affinity chromatography uses molecules, called effectors, to which the product has high and specific affinity, e.g., using an antibody (effector) for the purification of the antigen to which it is specific.

The effector is immobilized on a water insoluble carrier, which is packed in a column through which the mixture is passed. The effector binds only to the molecules for which it is specific and retains it in the column; it is later recovered by elution using a buffer solution of a specified pH.