The bacteria are grown on complex media based on maize or potato starch supplemented with soybean meal or corn steep liquor (medium has 20% dry matter). The fermentation is carried out for about 5 days, then the broth is chilled and the cells and solids are removed by flocculation.
The enzyme is extracellular, and is recovered from the broth; it is always stabilized with Ca2+ ions. Its applications are: dextrin production, first stage in glucose manufacture, in brewing and bakery, for removal of starch in textile manufacture, etc.
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