Culture Media - Inoculum preparation media are quite different from production media. These media are designed for rapid microbial growth, and little or no product accumulation will normally occur. Many production processes depend on inducible enzymes.
In all such cases, the appropriate inducers must be included either in all the stages or at least in the final stages of inoculum development. This will ensure the presence of the concerned inducible enzymes at high levels for the production to start immediately after inoculation.