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Extraction Process - The process of recovering a compound or a group of compounds from a mixture or from cells into a solvent phase is called extraction. Extraction usually achieves both separation as well as concentration of the product.

It is especially useful for the recovery of lipophilic substances, and in antibiotic recovery; it is often an early step after cell separation.

Liquid-Liquid Extraction
It employs two immiscible liquids into which the product is differentially soluble.
Usually successively smaller volumes of the solvent are used for repeated extraction of a giver back-extraction also tends to increase the selectivity of extra extraction may be performed in a single step, by multi-stage par extraction, or by counter-current extraction (most complex effective).

Whole Broth (Medium + Cells) Extraction
It should be used wherever possible since it reduces the number of steps as well as product loss. The effectiveness of extraction may, however, be reduced due to the presence of cells.

Aqueous Multiphase Extraction
It is used for separation of enzymes from cells/cell debris. The enzymes are extracted in an aqueous polyethylene glycol-dextran mixture, which form separate phases. Recovery of enzymes from these phases is rather easy and free from some of the difficulties encountered in centrifugation.