Whole Broth (Medium + Cells) Extraction
It should be used wherever possible since it reduces the number of steps as well as product loss. The effectiveness of extraction may, however, be reduced due to the presence of cells.
Aqueous Multiphase Extraction
It is used for separation of enzymes from cells/cell debris. The enzymes are extracted in an aqueous polyethylene glycol-dextran mixture, which form separate phases. Recovery of enzymes from these phases is rather easy and free from some of the difficulties encountered in centrifugation.
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