Fermentation -
Inoculum is developed in several stages as is the case with other fermentations. The organism may then be multiplied in one or more seed tank stages. The medium used for these stages is rather rich to support good growth.
The seed tank culture is finally inoculated into the production stage fermenter (25,000-100,000 1).The production medium is almost always complex and is devised to maximise yields of the enzyme.
Appropriate measures must be employed to ensure contamination control.
Biomass of the organism begins to increase early during fermentation, while enzyme activity shows significant increases about midway through the fermentation; the end of fermentation is signaled by cessation of enzyme synthesis.
