Industrial Microbiology

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 Industrial Microbiology

  Microbial Products of Potential Importance

  Microbes History

  Isolation and Screening of Microorganisms

  Isolation of Microorganisms

  Screening of Microorganisms for New Products

 Inoculum Development

  Culture Media

  Contamination

  Sterilization

  Common Contaminants

  Sterilization Procedures

  Strain Improvement

  Mutant Selection

  Selective Isolation of Mutants

  Different Approaches in Utilization of Mutation and Genetic Recombination for Strain Improvement

  Bioreactors

  Solid State Fermentation

  Anaerobic Fermentation

  Aerobic Fermentation

  Immobilized Cell Bioreactors

  Bioreactor Media

  Downstream Processing

  Separation of Particles

  Antibiotic Preparation

 List of Antibiotics Produced Commercially

  Media Used in the Different Stages of Antibiotic Production

  Enzymes

  List of Some Enzymes Used for Commercial Activities

  Fermentation

  Biosensors

  Isolation and Purification

  α - Amylase

  Amyloglucosidase

  Glucose Isomerase

  Biotransformation

  Biotransformation of D-Sorbitol to L-Sorbose

  Biotransformation of Antibiotics

  Recovery of Metals

  Desulphurisation of Coal

  Advantages of Using Microbes for Ore Leaching

  Disadvantages of Using Microbes for Ore Leaching

  Applications of Microbial Ore Leaching

  Bioinsecticides

  Bioherbicides

  List of Biocontrol Agents used against insects, Pathogens and Weeds

  Disease Control

 Comparison between Biopesticides and Chemical Pesticides

 Advantages and Limitations of Biopesticides and Chemical Pesticides

  Important Microorganism with Practical Application as Biofertilizers

  Rhizobium spp.

  Azotobacter and Azospirillum

  Blue Green Algae and Azolla

  Phosphate Solubilizing Microorganisms

  Advantages and Disadvantages of Biofertilizers

  Genetically Engineered Microbes - GEMS

Industrial Microbiology

Industrial Microbiology - Use of microbes to obtain a product or service of economic value constitutes industrial microbiology. Any process mediated by or involving microorganisms in which a product of economic value is obtained is called fermentation (Casida, Jr., 1968). The terms industrial microbiology and fermentation are virtually synonymous in their scope, objectives and activities.

The microbial product may be microbial cells (living or dead), microbial biomass, and components of microbial cells, intracellular or extracellular enzymes or chemicals produced by the microbes utilizing the medium constituents or the provided substrate.

The services generated by microorganisms range from the degradation of organic wastes, detoxification of industrial wastes and toxic compounds, to the degradation of petroleum to manage oil spills, etc. Industrial microbiology also encompasses activities like production of biocontrol agents, inoculants used as biofertilizers, etc.

Obviously, the scope and activities of industrial microbiology are too extensive to be covered in any detail in a book like this scope; therefore, the coverage in this chapter remains generalized and rather elementary.

The activities in industrial microbiology begin with the isolation of microorganisms from nature, their screening for product formation, improvement of product yields, maintenance of cultures, mass culture using bioreactors, and usually end with the recovery of products and their purification.

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  Identification of Metabolites

  Sample of the Screening Approaches Used for the Detection of Microbial Isolates Producing Useful Products

  Valuable Products from Microorganisms

  Recombination

  Sexual Reproduction

  Protoplast Fusion

  Recombinant DNA Technology

  Types of Changes in Metabolite Production Induced by Recombinant DNA Technology OR Genetic Engineering

  Process Development

  Shake Flask Fermentation

  Scale up of Process

  Disintegartion of Cells

  Extraction Process

  Concentration of Product

  Purification of Product

  Chromatography Methods

  Affinity Chromatography

  Drying of Products

  In Situ Recovery of Products

  Uses of Vacuum

  Two Phase Systems

  Dialysis

  Industrial Microboilogy Applications

  Metabolite Production

  Acetone Butanol Production

  Industrial Alcohol Production