Shake cultures are a convenient method of growing microorganisms in submerged cultures under aerobic conditions created by shaking; it is a small scale equivalent of stirred tank bioreactor. Both the devices are extensively used with filamentous microorganisms and, often, with other types of micro organisms as well.
Usually, complex media are used for shake flask cultures, but the objective is to devise a synthetic medium for the fermentation process. Studies on inoculum size, temperature, agitation, nutrition are initially done using these cultures to monitor their influences on growth and product formation.
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