Sterilization,Living Cells,Sterilization Procedures,Culture Flask

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	Sterilization - Sterilization is the process of inactivating or removing all living organisms from a substance or surface. In concept, it is regarded as absolute in that all living cells must be inactivated / removed, usually in a single step at the given time. But in practice, the success of sterilization procedures is only a probability.Therefore, the probability of a cell escaping inactivation/filtration does exist although it is usually very small.
When a closed system is sterilized once, it remains so indefinitely since it has no openings for the entry of microorganisms. But most fermentation vessels are open systems; such systems are initially sterilized and must be kept sterilized by ensuring the removal of living cells at their entry points, e.g., the cotton plug of a culture flask.