Chemcial Synthesis of tRNA Genes -As outlined above, before one can start the chemical synthesis of a gene, the structure of gene should be known. The structure of a gene earlier could not be worked out by direct chemical analysis, since there were no means for isolating a gene.

The structure of gene could, therefore, be inferred only from its product. For instance, if a gene is responsible for giving rise to a polypeptide chain and the structure of this chain is known, then from the genetic code dictionary, structure of the gene could be easily inferred.