Isolation of Genes Which are Tissue Specific in Expression, mRNA Molecules, Erythrocytes, Oviduct, Globin, Somatic Embryos

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	Isolation of Genes Which are Tissue Specific in Expression - It is much easier to isolate genes which are expressed in specific tissues. For instance, genes for storage proteins are expressed only in developing seeds, ovalbumin gene is expressed in oviduct or globin gene is expressed in erythrocytes.
Such genes can be easily isolated because mRNA extracted from these specific tissues will either exclusively belong to the gene of interest or it will be rich in this species of mRNA. Other mRNA molecules in minor quantities can be eliminated, since these can be identified through their isolation from tissues where this gene is silent. This strategy which is outlined was actually followed for isolation and cloned of carrot genes expressed during the development of the somatic embryos and also for the isolation of several genes for storage proteins in crop plants.