Maxam and Gilbert's Chemical Degradation Method - In this method, following steps are involved:

(i) Label the 3'ends of DNA with 32p.

(ii) Separate two strands, both labelled at 3'ends.

(iii) Divide the mixture in four samples, each treated with a different reagent having the property of destroying either only G, or only C, or 'A and G' or 'T and C. The concentration of reagent is so adjusted that 50% of target base is destroyed, so that fragments of different sizes having 32p are produced.

(iv) Electrophoreses each of the four samples in four different lanes of the gel.

(v) Autoradiograph the gel and determine the sequence from positions of bands in four lanes.