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Synthesis of Genes for Yeast Alanyl tRNA - As readers may know, structures of large number of tRNA molecules are now known.

The first tRNA whose structure could be known was yeast alanyl tRNA. R.W. Holley who died in early 1993 and his coworkers (in 1965) gave the detailed structure of yeast alanyl tRNA. This information was immediately used by H.G. Khorana and his coworkers to deduce the structure of the gene for yeast alanyl tRNA.

The structure of this gene would obviously be such that one of the two strands of DNA (gene) would be complementary to base sequence of yeast alanyl tRNA.

The other strand would then automatically have the same sequence as in tRNA except that in place of uracil of tRNA there would be thymine in DNA.