Synthesis of Oligonucleotides, Pentannucleotides, Icosanucleotides, Nucleotides

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	Synthesis of Oligonucleotides - Fifteen oligonucleotides ranging from pentanucleotide (5 bases) to an icosanucleotide (20 bases) were synthesized. Synthesis was conducted throl1.agh condensation between hydroxyl group at the 3' position of the nucleotide and phosphate group at 5' position of the second nucleotide.
In order to bring about condensation, all other functional groups, not taking, part in condensation was protected using specific protective groups. After protecting the groups, reaction between a nucleotide with protected 5' end and another nucleotide with protected 3' end proceed. Subsequently condensation was also brought about between groups of two, three or four nucleotides.