Use of RFLP - Restriction Fragment Length Polymorphism Maps OR Chromosome Walking for Gene Isolation,RELP Maps,Isogenic Lines,Target Gene

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	Use of RFLP - Restriction Fragment Length Polymorphism Maps OR Chromosome Walking for Gene Isolation - In recent years, RFLP (restriction fragment length polymorphism) maps have been prepared in maize, tomato, rice, etc. Linkage of RFLP loci with genes of interest are also being worked out as an aid to plant breeding.
If RFLPs are known which are very close to the target gene (as may be done through RFLP maps or chromosome walking), then by locating the RFLPs an either side of the target gene, a long intervening DNA segment may be isolated. This fragment may be used for sub cloning leading to the isolation of the desired gene. If RFLPs are examined in two near isogenic lines (NTLs) differing far a single gene, the difference in RFLP maps will help in locating the position of gene on molecular map which can then be utilized far isolation of the gene.