Use of Heterogenous Probes, Oligonucleotidies, DNA, Two Dimensional Gel Electrophoresis, Genomic, Allele, Nicotiana Alata

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	Use of Heterogenous Probes - We have already discussed the synthesis of cDNA probes above. However if protein, purified using the technique of two dimensional gel electrophoresis, is used for micro sequencing of 5-15 consecutive amino acids, this information can be used for the synthesis of oligonucleotides (using automated DNA synthesizers).
These oligonucleotides may then be directly utilized for screening of cDNA or genomic libraries for isolation of specific genes. These can also be used for designing primers for the amplification of a gene in DNA extracted from a tissue. A 32 kilo Dalton (KD) glycoprotein (isolated from style) associated with the S2 incompatibility allele of Nicotiana alata, was used for synthesis of an oligonucleotide leading to the isolation of a related gene.