Heterologus
Activities
Recruited
to
Alter
Metabolite
and
Protein
End
Products -
| Host Organism | Original metabolite | Heterologus enzyme added (source organism) | New product |
Erwinia herbicol |
2, 5-DKG | 2, 5-DKG reductase (Corynebacterium) | 2 KLG |
| Acremonium chrysogenum |
Cephalosporim C | (i) D-amino acid oxidase (Fusarium salani) |
7 ACA |
| (ii) Cephalosporin acylase (Pseudomonas diminuta) | |||
| CHO cells | Terminal β galactosyl residues in N- acetyllactosamine sequences | β -Galactoside α 2, 6 sialyl transferase (rat) | sinalyl α 2,6 galactosyl linkages |
| Mouse L cells | Unsubstituted type II, N-acetyllactosamine glycoconjugate end groups | G-Dp-L-fucose : β-D- galactoside 2- α-L- fucosyl transferase (A431 human cell line) | H fucosyl α 1-->2 galactosyl linkages |