Limitations
Due
to
Lack
of
Knowledge
about
Concentrations
of
Intracellular
Proteins
and
Metabolites -
Different analytical methods are often used for determining either the concentrations of cellular proteins and metabolites, or the rates of reactions, or the fluxes in specific biochemical pathways. These include the following:
(i) two dimensional electrophoresis,
(ii) use of isotopically labelled precursors, and
(iii) nuclear magnetic resonance (NMR) spectroscopy. Despite the availability of these analytical methods, the time and instrumentation required to evaluate metabolite concentrations limits rational metabolic engineering.
