Metabolic Control Theory and Metabolic Engineering - Theoretical development of metabolic control theory has been a significant advance in metabolic engineering. It allows calculation of sensitivity which gives estimates of 'flux control coefficients' to be estimated as follows:

The sensitivity of flux (through the pathway) to other parameters in kinetic expressions for each enzyme can also be estimated under different growth conditions (temperature, pH, etc.). This will provide guidance for useful metabolic engineering leading to acceleration of the pathway.