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Modification of Metabolic Regulation - A metabolically engineered baker's yeast was developed through modification of the regulation of expression of genes for maltose permease and maltase. This recombinant yeast was intended to reduce the time "for leavening of sweet doughs.

These genes are glucose sensitive, so that glucose represses their expression. Therefore, one would like that glucose uptake and catabolism should be associated with that of maltose.

Same constitutive yeast promoters were used for separate genes responsible for uptake and catabolism of glucose and maltose, so that simultaneous uptake (by permease enzyme) and catabolism of both sugars is possible.

In yeast, a futile and accessory metabolic cycle was created, which led to increased CO2 generation. Increase in CO2 is favoured, because CO2 in yeast consumes ATP, which otherwise inhibits the enzymes (phospho fructokinase or PFK, and pyruvate kinase) used in sugar catabolism.

Similarly, alkylbenzoate degradation pathway encoded on a Pseudomonas plasmid was modified (through mutation), so that a promoter Pm for the operon (metaoperon) meant for degradation of 4-ethylbenzoate (a pollutant) was activated by the same compound (4- ethylbenzoate). The metabolically engineered Pseudomonas can thus be used for the processing of 4- ethylbenzoate.

 

  

 
 

 
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