Redirecting Metabolite Flow - In a branched biosynthetic pathway, there are always several forks; where intermediates can enter alternative pathways. At such forks; resources (substrate, enzyme, transport system, ribosomes, etc.) are distributed equally or unequally to two or more processes.

When we are interested in one of the several possible products, at a fork the desired route or process can be made a priority, so that resources are mainly channelized to this route and the traffic to other routes is minimized without decreasing cell viability.

This can be achieved by several methods including the following:

(i) use of cloned genes inhibiting feedback inhibition by the end product,

(ii) use of mutant ribosomal RNA and

(iii) use of positive regulatory sequences.