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Herbicide Tolerant Plants - The presence of weeds in a crop field reduces the yield. Weed killers or herbicides are not very selective and their current use relies on differential uptake between the weed and the crop plant or on application of the herbicide before planting a field, altering the food content of plants. With the ability to introduce DNA into plants, researchers are trying to create herbicide-tolerant crops by three strategies.

1. Increasing the level of the target enzyme for a particular herbicide.

2. Expressing a mutant enzyme that is not affected by the compound.

3. Expressing an enzyme that detoxifies the herbicide.

Of the large number of herbicides in use today, only a few of the cellular targets have been characterized. The first strategy is to clone the cellular target genes into the plant so that they are produced in large amounts.

Another strategy for creating herbicide tolerant plants has used mutant forms of bacterial EPSPS enzymes. Genes encoding these mutant enzymes have been doned from glycophosphate resistant bacteria and expressed in plants. These enzymes have lesser inhibition effect by herbicides.

The third strategy for creating herbicide tolerant plants is by transgenic expression of enzymes that convert the herbicide to a form that is not toxic to the plant. Some plants have developed their own detoxifying system for certain herbicides. But these activities in plants are encoded by a complex set of genes that has not yet fully characterized.