Human Growth Hormones - hgH Production by Using Molecular Biotechnology Techniques -Children with hypopituitary dwarfism do not grow in size in normal proportions like children with normal pituitary function because their bodies make too little growth hormone.

They are destined to be dwarfs unless they are treated with hGH. Growth hormone from other animals may not help. Moreover, early preparations of hGH were contaminated with pyrogens or other contaminants.

Growth hormone is a 191 amino acid protein that is produced in the pituitary gland and regulates growth and development. The production of hGH was achieved by constructing a hybrid gene from the natural hGH cDNA and synthetic oligonucleotides that encode the amino terminus of the mature form of the protein. This coding sequence was ligated into a plasmid adjacent to a bacterial promoter.