Molecular Genetic Maps in Mouse,Recombinant Inbred,Genome,Molecular Markers,Musculus Genome Organization

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	Molecular Genetic Maps in Mouse - The genetic maps in mouse have been prepared essentially through the use of the following material: (i) non-identical laboratory strains were used for crosses leading to the production of F2 and backcross segregating populations; (ii) recombinant inbred (RI) lines were used either for crosses or like F2 individuals with the parents which differed for specific marker loci; (iii) inter specific backcrosses (Mus musculus x M. spretus) were also sterilized, since M. spretus exhibited high level of genetic divergence with musculus.
The mouse has a genome, which is as big as that of humans. RFLPs, APDs and SSRs (microsatellites) were used as molecular markers. In 1989, a linkage map with 965 loci was presented. This map included phenotypic markers, biochemical markers, cloned gene's, and anonymous DNA markers subsequently in 1991,600 loci were positioned on the map using interspecific crosses, which gave a resolution of 3cM. More than 300 SR loci were also mapped in 1992. It is expected that by 1995, a mouse linkage an with a resolution under 1cM will be available. Most of this work will be clone in USA, by MUGO (Mus musculus Genome Organization).