Properties
of
RFLPs
(
Restriction
Fragment
Length
Polymorphism)
and
RAPD
(Random
Amplified
Polymorphic
DNAs) -
| Properties |
RFLPs |
RAPDs |
| 1. Distribution |
Ubiquitous |
Ubiquitous |
| 2. Inheritance |
Simple mendelian,stable, co dominant |
Simple mendelian,stable, dominant/null |
| 3. Level of polymarphism |
High |
High |
| 4. Detect alllelic variant? |
Yes |
No |
| 5.No.of loci detected? |
1-3 |
1-10 |
| 6. Part of genome surveyed |
Generally low-copy, coding regions |
Whole genome |
| 7. Detectable in all tissues? |
Yes |
Yes |
| 8. Technical difficulty |
Intermediate |
Low |
| 9. Reliability |
High |
Intermediate/high (occasional spurious products) |
| 10. Quality of DNA required |
Relatively pure |
Crude |
| 11. Quantity of DNA required |
2-10 μg |
10-50 ng |
| 12. Use radioisotopes? |
Yes |
No |
| 13. Type of probes required |
Species-specific low copy gDNA or cDNA clones |
Randon 9- or 10 mer oligonucleotides |
| 14. Recurring cost |
High |
High |
|
