Steps
Involved
in
RFLPs
(
Restriction
Fragment
Length
Polymorphism)
and
RAPD
(Random
Amplified
Polymorphic
DNAs)
Analysis -
| RFLP |
RAPD |
| 1. Extration of DNA |
1.Extration of DNA |
| 2.DNA digestion with restriction enzyme(s) |
2.Amplification of DNA by PCR using random primers |
| 3. Separation of DNA fragments on agarose gel |
3. Separation of amplified DNA on agarose gel |
| 4. Transfer of DNA fragments southern transfers |
4. Visualization of markers on the gel and photography of the gel |
| 5. Hybridization of southern transfers with probes (s) |
|
| 6. Detection of probe using autoradiography or using a non radioactive system. |
|
|
