In the form of biochemical markers, isozymes have been used, but their use is limited due to lack of polymorphism and a small number, of loci and alleles available for analysis. The use of RFLPs for characterization overcomes this limitation, since they provide substantial polymorphism and they can be unlimited in number.
The only disadvantage of using RFLPs for characterization of genetic resources is the cost of material and labour, which may gradually be minimized. Developments like PCR (polymerase chain reaction) and RAPD (random amplified polymorphic DNA, pronouced as 'rapid') will make the use of molecular markers more convenient and more economic.
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