After hybridization the membrane or slide may be washed and the membrane or the slide is transferred into in to detection buffer containing 20 micro g/ml of antidigoxigenin fluorescein and 5% (w/v) BSA (bovine serum albumin).
The system is incubated for that 37°C, and then the membrane slide washed in detection buffer three times (8 min each at 37°C). Alkaline phosphatase activity can be detected using 0 .17 mg/ml BCIP (5-bromo-4-chloro-3 indolyl phosphate) and 0.33 mg/ml NBL (nitroblue tetrazolium) as dye substrate
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