(ii) The genomic or cDNA library can be screened through colony hybridization or plaque hybridization (see later) to identify and isolate the clone carrying the desired sequence; sometimes the step (i) is not used and complete genomic/cDNA libraries can be prepared and used in step (ii);
(iii) The identified clone should carry the gene or sequence of interest, so that the clone can be multiplied and the sequence or gene retrieved according to standard procedure.
|
