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Genetic Material -

Several lines of indirect evidence have long suggested that DNA contains the genetic information of the living organism. Results obtained from several different experimental procedures showed that most of the DNA is located in the chromosomes, whereas the RNA and proteins are also abundant in the cytoplasm.

Moreover, a precise correlation exists between the amount of DNA per cell and the number of sets of chromosomes per cell. That is, most somatic cells of diploid organisms for example, contain exactly twice the amount of DNA as the haploid germ cells or gametes of the same species. The molecular composition of the DNA in all the different cells of an organism is the same, whereas the composition of RNA and protein varies both qualitatively and quantitatively from one cell type to another.

Direct evidence showing that the genetic material is DNA rather than protein or RNA was published by Avery, MacLeed and McCarty in 1944. They demonstrated that the component of the cell responsible for the phenomenon of transformation in the bacterium Diplococcus pneumoniae was DNA.

The genetic information of all living organisms, except the RNA viruses, are stored in DNA. Nucleic acids, originally called 'nuclein' because they were isolated from cell nuclei by F. Miescher, in 1869, are macromolecules composed of repeating subunits called nucleotides.

Each nucleotide is composed of

(1) a phosphate group,
(2)a five-carbon sugar, and
(3) a cyclic nitrogen are commonly found in DNA: adenine, guanine, thymine, and cytosine.

RNA also usually contains adenine, guanine, and cytosine but has a different base, uracil, in place of thymine. Adenine and guanine are double-ring bases called purines; cytocine, thymine, and uracil are single ring bases called pyrimidines. Hence both DNA and RNA contain four different subunits or nucleotides two purine nucleotides and two pyrimidine nucleotides. RNA usually exists as a single stranded polymer composed of a long sequence of nucleotides. DNA, however, has one very important additional level of organization; it is usually a double stranded molecule.

 The correct structure of DNA was first deduced by Watson and Crick in 1953 ,as a double helix in which the two polynucleotide chains are coiled about one another in a spiral. Each polynucleotide chain consists of a sequence of nucleotides linked together by phosphodiester bonds, joining adjacent deoxyribose moieties

The two polynucleotide strands are held together in their helical configuration by hydrogen bonding between bases in opposing strands, the resultant base pairs being stacked between the two chains perpendicular to the axis of the molecule like the steps of a spiral staircase. The base pairing is specific adenine is always paired with thymine and guanine is always paired with cytosine. Thus all base pairs consist of one purine and one pyrimidine. The specificity of base pairing results from the hydrogen bonding capacities of the bases in their normal configuration. In their most common structural configuration, adenine and thymine form two hydrogen bonds and guanine and cytosine form three

Once the sequence of bases on one strand of a DNA in a double helix is known, the sequence of bases in the other strand is also known because of specific base pairing. The two strands of a DNA in a double helix are thus said to be complementary.

It is this complementarity that makes the DNA unique in transmitting genetic information. DNA molecules are highly stable. This is partly due to the large number of hydrogen bonds between the base pairs and hydrophobic bonds between the stacked base pairs,Nearly all of the DNA molecules present in the aqueous protoplasm of living cells almost certainly exist in the Watson and Crick double helix form described above.

This is the B-form of DNA. However, the structure of DNA molecules changes as a function of their environment. The exact conformation of a given DNA molecule or segment of a DNA molecule will depend on the nature of the molecule with which it is interacting.

Certain DNA sequences have been shown to exist in a unique, left handed double helical form called Z-DNA (Z for the zigzag path of the sugar phosphate backbones of the structure). In a high concentration of salts or in a dehydrated state DNA exists in the A-form which has 11 nucleotide pairs per turn.

It is very unlikely that DNA molecules ever exist in the A-form in VIVO. The helices of the A- and B-forms of DNA are wound clockwise. They can undergo conformational shifts from the B-form to the Z-form and vice versa. Certain regulatory proteins are bound to the Z-form or B-form of a DNA sequence and cause it to shift to the B-form or Z-form.