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Nuclear Genome -

A genome is the complete set of chromosomes found in each nucleus of a given species which contains the entire genetic material. The nuclear genome is the largest in the plant cell, both in terms of picograms of DNA and in number of genes encoded (complexity). Eukaryotic nuclear genomes can be distinguished from organelle and prokaryotic genomes by size and complexity. A typical higher plant genome, for example, contains about 5 x 109 base pairs of DNA per haploid set of chromosomes. This is about 30,000 times as much DNA as in a single chloroplast genome and some 10,000 times as much as in a moderately sized plant mitochondrial genome. It is also 1000 times more than that of bacterial DNA present in Escherichia coli.

The typical plant genome with 5 x 109 base pairs of DNA would be about three metres long if the entire DNA were to be laid out in a straight line. Chromosomes are composed of two types of large organic molecules (macromolecules) called proteins and nucleic acids.

Nucleic acids are of two types: deoxyribonucleic acids. (DNA) and ribonucleic acids (RNA).For many years scientists disputed which of these three macromolecules (proteins, RNA and DNA) carries genetic information.

During the 1940s and early 1950s several elegant experiments were carried out which clearly established that genetic information resides in the nucleic acids and not in the proteins. More specifically, these experiments showed that genetic information resides in DNA. (In a few simple viruses, however, RNA carries the genetic information; these particular viruses contain no DNA.) Nuclear DNA is packaged into chromosomes along with histones and nonhistone proteins, all of which play important roles in gene expression.

These various components are held together to form chromatin by both hydrophobic and electrostatic forces. While the DNA encodes the genetic information, the proteins are involved in controlling packaging of DNA and in regulating its availability for transcription. Although the structure of eukaryotic chromatin has been fairly well characterized, the roles of various individual proteins in chromatin structure and gene regulation have yet to be elucidated.