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Plant Gene Structure -

Plant ribosomal RNA genes and a number of other structural genes from a variety of species have now been analyzed in considerable detail. In common with many animal genes, some plant gene sequences have been found to have their coding sequencers interrupted by introns or intervening sequences.

These introns are transcribed but not represented in mature mRNA and hence are not translated. No introns have been found in rRNA genes but they have been demonstrated in a number of other plant structural genes.

Introns and Exons: An intron is a noncoding section of a gene that is removed from RNA before translation in cells of higher organisms. An exon is the coding section of a gene. The coding sequences are interrupted by intervening noncoding sequences. Introns are detected as DNA sequences that lie within a gene yet do not appear in mature mRNA.

The best way to detect and characterize introns is by comparing the complete nucleotide sequences of DNA to the mRNA using genomic and cDNA clones. Cloned genomic DNA is annealed with RNA and the products examined under an electron microscope.

Since the intron sequences are not contained in the RNA, they will form single stranded loops in the heteroduplex molecule. If these loops are sufficiently large (more than 50 nucleotides in length), they can be seen and their approximate positions in the gene mapped from an electron micrograph.

The evolutionary and functional significance of an intron sequence has been much discussed. One of the most appealing hypotheses, originally put forward by W. Gilbert of Harvard University in 1981, is that the presence of introns is related to an evolutionary process by which new genes arise from recombination events that juxtapose previously existing genes or portions of genes.

Given the ability to splice transcripts from two or more DNA segments into a single mRNA, "compound" genes may form.The number of introns is highly variable in both plant and animal genes. There are a number of plant genes with no introns at all, such as the zein storage protein genes of maize, two other soybean protein genes, one rare class leaf gene and most cab genes (nuclear genes encoding the chlorophyll a/b-binding protein of the photosystem).

Other plant genes have two or three introns. Nucleotide sequence data for the French bean (Phaseolus) genes, soybean leghemoglobin genes, a soybean actin gene, soybean glycinin gene, a rare class leaf gene and genes for the small subunit of RuBP carboxylase, has shown that all these plant genes have two to three introns.

A few plant genes have larger numbers and or longer intron sequences. There are nine introns in the maize Adh genes and five introns in the phytochrome gene. Where intervening sequences are present, all plant genes are identical in the first and last two bases of the introns GT and AG respectively.

Conservation of the two dinucleotides at the intron/exon junctions in all eukaryotic genes suggests that similar RNA splicing mechanisms are involved. Comparisons of plant storage proteins with animal storage proteins have shown that plants have simpler intergenic structures than do animals.