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Agar Cultures - Agar-gelled medium is the most commonly used for experimental and some commercial activities, e.g., micro propagation. Generally, during experimentation, culture tubes of 25 x 150 mm are used for culture; here scaling up can be achieved by increasing the number of culture vessels.

These culture tubes are usually arranged in test tube baskets or stands, which are placed on culture racks. Further scaling up is afforded by the use of culture vessels, e.g., 38 X 200 mm culture tubes and, more particularly, 500 ml wide mouth bottles, magenta boxes, etc.

In case of bottles/magenta boxes, 2-4 separate pieces of the tissue inoculum may be placed in a single vessel. Half litre bottles are the most commonly used culture vessels in commercial micropropagation activities. Scaling up of agar cultures involves considerable labour since each vessel has to be handled individually during subculture, etc.