These plants were backcrossed to B. juncea thrice. In the progeny of third backcross, some plants were identified that were able to restore fertility in a cytoplasmic male sterile (CMS) line having T. ballii cytoplasm. The CMS line itself was obtained by backcrossing five times the symmetric hybrid produced by fusion of B. juncea and T. ballii protoplasts to the B. juncea fusion parent.
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