Comparison
of
RFLP
and
RAPD
Markers
for
Genome
Mapping
in
Plants -
Feature |
RFLP |
RAPDs |
| Inheritance pattern |
Codominant |
Dominant |
| Detection of multiple alleles of a marker |
Yes |
No |
| Quality of DNA needed for study |
Pure |
Crude |
| Amount of DNA needed |
2 - 10 µg |
> 10 ng |
Radioisotopes |
Must be used (in probes) |
Not used |
Restriction enzymes |
Must be used |
Not used |
| Type of probe used |
Species specific probes, generally, low copy genomic or cDNA |
Random base sequence,9-12 base nucleotides |
Time required |
About 5-times more than RAPDs |
1/5 of that for RFLPs |
|
