Comparison
of
Three
Approaches
for
In
Vitro
Germplasm
Conservation -
| Feature |
Cryopreservation |
Slow-growth |
DNA clones |
| Tissue/organe conserved |
Shoot-tips, zygotic or somatic embryos, cells, protoplasts |
Slow growing shoots |
DNA pieces as phage clones |
| Metabolic activity |
Nil |
Slow |
Nil |
| Storage temperature |
- 196° C |
4-9 or ~ 15° C |
4° C; in lyophylised state |
| Storage in |
Liquid nitrogen refrigerators |
Ordinary refrigerators |
Ordinary refrigerator/deep freeze |
| Attention needed during storage |
Replenishing liquid nitrogen |
Subculture every 6-36 months |
Virtually nil |
| Risks |
Loss in viability with time |
Contamination |
Virtually nil |
| Applicable to |
All species amenable to tissue culture |
All species amenable to tissue culture |
Conservation of valuable genes |
| Sophistication |
Sophisticated |
Less Sophisticat |
Highly Sophisticated |
| Cost involved |
Costly equipments required |
Standard tissue culture facilities needed |
Very costly as it is based on recombinant DNA technology |
|
