Development of High Producing Clones for Improving Biochemical Production - Biochemical content of cell cultures depends on the plant species, the genotype or strain of the species, the specific plant of a strain, and even the explant from which they are derived. In general, high producing plants yield high producing cultures.Therefore, cell cultures must be started from the highest producing plants of the species in question.

In addition, different plant species producing a given biochemical may differ markedly in the site of biochemical accumulation. For example, cell cultures of Coptis japonica accumulate berberine within cell vacuoles, while those of Thalictrum minus secrete most of the alkaloid into the culture medium where it crystallizes as nitrate or chloride salt.