Low concentrations (50-250 mg/l) of elicitors added to the culture medium dramatically enhance secondary metabolite production by plant cells. Chitosan is a very effective elicitor in several systems. Cell wall pectins (100 mg/l of medium), isolated from suspension cultures of Morinda citrifolia increased anthraquinone production by M. citrifolia cell suspensions to 5.6 times that of control after a 14-day incubation.
Biotic elicitors bind to a receptor protein in plasma membrane, which ultimately leads to the accumulation of secondary metabolites. The responses to elicitor polysaccharides seems to be similar to the defense response to micro-organism
invasion.
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