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Elicitors for Improving Biochemical Production - Some molecules stimulate the production of secondary metabolites; these are called elicitors, and the phenomenon is known as elicitation. Elicitors produced within plant cells are termed as endogenous elicitors, while those produced by microorganisms are called exogenous elicitors.

Elicitors of plant origin are polysaccharides derived fryuhom-cell walls, e.g., pectin, pectic acid, cellulose, while those of microorganism origin are cell wall components like chitin, chitosan or glucans. These elicitors are of biological origin; hence they are called biotic elicitors.

Low concentrations (50-250 mg/l) of elicitors added to the culture medium dramatically enhance secondary metabolite production by plant cells. Chitosan is a very effective elicitor in several systems. Cell wall pectins (100 mg/l of medium), isolated from suspension cultures of Morinda citrifolia increased anthraquinone production by M. citrifolia cell suspensions to 5.6 times that of control after a 14-day incubation.

Biotic elicitors bind to a receptor protein in plasma membrane, which ultimately leads to the accumulation of secondary metabolites. The responses to elicitor polysaccharides seems to be similar to the defense response to micro-organism invasion.