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Hair Root Cultures - Hairy roots are induced by transforming plant cells with Agrobacterium rhizogenes. These roots can be grown indefinitely, and in large fermenters at growth rates comparable to that of suspension cultures. Hairy roots synthesize secondary metabolites and at levels characteristic of the roots of parent plants.

A. rhizogenes may be used to transform leaf discs, other organs or even protoplasts. The hairy roots so obtained are excised and used to initiate root cultures; hairy root cultures are easily developed in most dicot plants.

Hairy root cultures
(i) are suitable for production of all root derived biochemicals,
(ii) are faster growing, higher producing and much easier to maintain than untransformed root cultures,
(iii) present no risk of instability common with cell cultures,
(iv) in some cases a portion of the product is released in the medium, and
(v) provide opportunities for isolation of stable high producing hairy root lines by exploiting somaclonal variations. Hairy root cultures are quite promising, particularly in case of tropane alkaloids.