Limitations of Genetic Transformation - 1. Techniques for protoplast isolation, culture and fusion are not available for many important crop species like many cereals and pulses.
2. In many cases, chromosome elimination occurs from somatic hybrids leading to asymmetric hybrids. Such hybrids may be useful, but there is no control on chromosome elimination.
3. Many somatic hybrids show genetic instability, which may be an inherent feature of some species combinations.
4. Many somatic hybrids either do not regenerate or give rise to sterile regenerants. Such hybrids are useless for crop improvement.