6. A sophisticated tissue culture laboratory and a dependable greenhouse are essential for success.
7. Specialized skill for carrying out the various operations is required.
8. Plants other than haploids are often recovered from anther cultures/interspecific hybrids; this necessitates cytological analyses.
9. Occurrence of grametoclonal variation may limit the usefulness of pollen embryos for genetic transformation/gene transfer.
10. High frequency of albinos are produced in anther cultures of monocots, especially cereals.
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