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Limitations of Haploids - 1. In many crops, the application of this technique is not yet possible since the technique for haploid production is not available.

2. In many other crops, its application is not feasible because large numbers of haploid plants are not easily obtained.

3. High cost of obtaining haploids and doubled haploids is still a major problem.

4. Sometimes, deleterious mutations may be induced during the in vitro phase.

5. A nonrandom recovery of haploids may reduce the spectrum of variability recovered.

6. A sophisticated tissue culture laboratory and a dependable greenhouse are essential for success.

7. Specialized skill for carrying out the various operations is required.

8. Plants other than haploids are often recovered from anther cultures/interspecific hybrids; this necessitates cytological analyses.

9. Occurrence of grametoclonal variation may limit the usefulness of pollen embryos for genetic transformation/gene transfer.

10. High frequency of albinos are produced in anther cultures of monocots, especially cereals.