Similarly, for large scale somatic embryo (SE) production, the first reactor may be in continuous mode, while the second may be of batch type. The continuous first stage reactor provides the conditions necessary for rapid proliferation of embryogenic cells (but not for SE development).
The cell mass from this bioreactor serves as a continuous source of inoculum for the second stage batch type bioreactor, which has conditions necessary for embryo development and maturation (but not for cell proliferation).The use of continuous first stage bioreactor:
(i) avoids the time, labour and cost needed for cleaning, etc. of a batch reactor between two runs,
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