Problems
in
Large
Scale
Culture
of
Plant
Cells - Large scale culture of plant cells for commercial applications like biochemical, artificial seed, and etc. production presents several problems.
1. Plant cells have much slower growth rates than bacteria and fungi, therefore, larger reactors and longer fermentation times are necessary.
2. The long fermentation time increases the risk of contamination.
3. Plant cells are rather sensitive to shear.
4. Plant cells show cytogenetic, genetic and epigenetic variations during culture.
5. In case of biochemical production, often conditions favouring rapid growth may suppress biochemical formation and vice versa.