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Importance of Plant Tissue Culture in Biotechnology-

Cell suspension culture in liquid medium is a relatively young field of biotechnology. This technology involves the large scale culture of isolated plant cells under conditions which induce them to synthesize the natural secondary metabolites characteristic of the parent plants from which they were obtained.

The techniques of callus culture and cell suspension culture have been reviewed in recent years, in particular from the viewpoint of studying biosynthesis and metabolism of steroids and cardiac glycosides. Biotech­nologists are also trying to increase the synthesis of natural compounds or new compounds by higher plant cell culture as a result of mixing or feeding transformable precursors in the culture medium. The metabolic process of transformation or conversion of such added precursors into a natural or new compound within the cell is known as, biotransformation. Biotechnologists are also trying to augment the synthesis of medicinally important alkaloids in culture by means of a fungal elicitor.

This means that cells are cultured in a liquid medium by adding the required quantity of a bacterial filter sterilized extract of certain fungi. Studies have shown that the fungal extract in certain cases helps to increase the synthesis 6f a desirable compound by the higher plant cell.

 Biotechnologists are also trying to modify the genetics of cultured cells in three ways:

(i) mutagenesis and selection of cell lines in cell suspension culture,
(ii) transplantation of foreign genetic material in protoplasts by means of genetic engineering, and
(iii) somatic hybridization by the fusion of distantly related plant protoplasts just to widen the genetic diversity of hybrids.

In the field of mutagenesis and selection of cell lines in vitro and the exploitation of totipotency, biotechnologists are trying to improve plants, for example, by producing crop species that are more resistant to drought, disease, poor soil conditions, chemical pesticides and herbicides.

Transplantation of foreign genes into protoplast has been achieved. Somatic hybridization by the fusion of protoplast brings together in a single plant, genes of different species too unrelated to allow mixing, thereby allow­ing creation of hybrid or cybrid plants for improvement of the crop species. Another goal of biotechnology using the plant tissue culture technique is to produce plants that would provide their own usable nitrogen.