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Plant Breeding and Plant Improvement -

Conventional breeding methods are the most widely used for crop improvement. But in certain situations these methods have to be supplemented with plant tissue culture techniques, either to increase their efficiency or to achieve an objective not possible through conventional methods.

Embryo culture is now routinely used in recovery of hybrid plants from distant crosses. Some examples are recovery' of hybrids from Hordeum vulgare x Agropyron repens and H. vulgare x Triticum sp. In the case of Triticale, a rare hybrid between Triticum and Secale develops viable seeds. But most of the tetraploid and hexaploid wheat carry two dominant genes, Kr1 and Kr2, which prevent seed development in crosses with Secale.

The hybrid seeds are minute, poorly developed and show very poor germination. By embryo culture, 50-70% hybrid seedlings have been obtained. Hybrid seedlings from T. aestivum x H. vulgare are not obtained. But in embryo culture when H. vulgare or T. aestivum (used as male) is crossed with H. bulbosum (used as female), the chromosome complement of H. bulbosum is eliminated from the developing embryo.

Most of the seedlings obtained from such crosses are haploid, having only one set of chromosomes, either from the H. vulgare or the T. aestivum parent. Embryo culture is also useful for propagation of orchids, shortening the breeding cycle and overcoming seed dormancy.

In meristem culture the shoot apical meristem along with some surrounding tissue is grown in vitro. This is used for clonal propagation and recovery of virus free plants and is potentially useful in germplasm exchange and long term storage of germplasm through freeze preservation.

Anther and pollen culture has potential application in a plant breeding and plant improvement program for the production of haploid as well as homozygous diploid plants. Year round rapid clonal propagation using plant tissue culture techniques has highlighted possibilities for new plant improvement techniques. Protoplast culture and somatic hybridization is a promising line for plant breeding and plant improvement techniques.

Another important approach is the mutation of tissue culture cells to produce a mutant line from which plants can be raised. Production of a mutant line is highly desirable for plant breeding. Callus cells, produced either from a vegetative cell or reproductive tissues, can be subjected to a range of mutagenic chemicals, e.g. N-nitroso-N-methyl urea, or ionizing radiations, e.g. gamma rays.,

The hope is that such treatment will effect permanent changes in the DNA pattern of some cells. Plants could be raised from the treated cultures and any mutant whole plants could be selected from the population either by physical differences or by metabolic/biochemical differences. Biochemical mutants could be selected for disease resistance, resis­tance to phytotoxin, improvement of nutritional quality, adaptation of plants to stress conditions, e.g. saline soils, and to increase the biosynthesis of plant products used for medicinal or industrial purposes.