|
|
Composition
of
Some
Plant
Tissue
Culture
Media -
| Compound |
Concentration (mg/l) |
| |
White's1 |
MS2 |
B53 |
| Inorganic |
|
|
|
NH4NO3 |
-- |
1650 |
-- |
| KNO3 |
80 |
1900 |
2527.5 |
| MgSO4.7H2O |
750 |
370 |
246.5 |
| KH2PO4 |
-- |
170 |
-- |
| (NH4)2SO4 |
-- |
-- |
134 |
| Ca(NO3)2.4H2O |
300 |
-- |
-- |
| Na2SO4 |
200 |
-- |
-- |
|
| NaH2PO4.H2O |
19 |
-- |
150 |
| KCI |
65 |
-- |
-- |
| CaCI2.2H2O |
-- |
440 |
150 |
| KI |
0.75 |
0.83 |
0.75 |
H3BO3 |
1.5 |
6.2 |
3 |
| MnSO4.4H2O |
5 |
22.3 |
-- |
| MnSO4.H2O |
-- |
-- |
10 |
| ZnSO4.7H2O |
3 |
8.6 |
2 |
|
| Na2MoO4.2H2O |
-- |
0.25 |
0.25 |
| MoO3 |
0.001 |
-- |
-- |
CuSO4.5H2O |
0.01 |
0.025 |
0.025 |
| CoCl2.6H2O |
-- |
0.025 |
0.025 |
| Fe2 (SO4)3 |
2.5 |
-- |
-- |
| FeSO4.7H2O |
-- |
27.8 |
-- |
| Na2.EDT A.2H2O |
-- |
37.3 |
-- |
| Sequestrene 330 Fe4 |
-- |
-- |
28 |
| Organic |
|
|
|
| (a) Vitamins |
|
|
|
| Inositol |
-- |
100 |
100 |
| Nicotinic acid |
0.05 |
0.5 |
1 |
|
|
| Pyridoxine HCl |
0.01 |
0.5 |
1 |
| Thiamine HCl |
0.01 |
0.1 |
10 |
| (b) Amino acid |
|
|
|
| Glycine |
3 |
2 |
-- |
| (c) Carbon source |
|
|
|
| Sucrose (g/l) |
20 |
30 |
20 |
| (d) Growth regulators |
As per need |
(Section 3.5.4) |
|
| Gelling agent (for callus cultures) |
|
|
|
| Agar |
Generally 6-8 g/l of the medium
Medium pH generally adjusted to 1N HCl or 1N NaOH |
|
| |
|
