Cryopreservation - Freezing and storage of tissue cultures in liquid nitrogen at -196°C would be of great value in the conservation of germplasm of those crops, which normally do not produce seeds, e.g., root and tuber crops, or where it may not be desirable to store seeds.

The preservation of cells, tissues and organs in liquid nitrogen is called cryopreservation, and the science pertaining to this activity is known as cryobiology. Many studies have been carried out on cryopreservation of plant cells and organs, and the approach appears to have considerable promise in germ plasma conservation.