Culture Medium for Pollen Embryogenesis - Medium requirements may vary with the species, the genotype, the age of donor plants and anthers, and the conditions under which the donor plants are grown. For example, pollen grains of Datura and tobacco produce embryos on an agar medium containing only 2-4% sucrose, while elaborate media e.g., N6 and Potato-2 media, had to be formulated for cereals. Sucrose is essential for anther cultures; the concentration may range from 3.% for barely to 6% for wheat and potato, but 2-3% sucrose is most commonly used. For most plant species, a complete tissue culture medium is required; MS, LS (Linsmaer and Skoog) and some other tissue culture media are generally used.